Cell Line Information:

Designations: RBL-2H3
Depositors: RP Siraganian
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: fibroblast
Source: peripheral blood
Strain: Wistar
Disease: basophilic leukemia
Cell Type: basophil; chemically induced
Cellular Products: histamine
Applications: transfection host
Receptors: FcERI (Fc of IgE)
Comments: RBL-2H3 is a basophilic leukemia cell line isolated and cloned in 1978 from Wistar rat basophilic cells that were maintained as tumors.
These cells have high affinity IgE receptors. They can be activated to secrete histamine and other mediators by aggregation of these receptors or with calcium ionophores. They have been used extensively to study FcERI and the biochemical pathways for secretion in mast cells. They have also been used extensively for studies of different aspects of secretion in cells including the role of changes in intracellular calcium, the activation of phospholipases, protein kinases and small G proteins.

The Transfection Reagent:

RBL-2H3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RBL-2H3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RBL-2H3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in RBL-2H3 Cells.
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Compatible with serum
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

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