Cell Line Information:
Cell Line Name: SF295
Patient"s Age: 67
Patient"s Sex: F
Prior Treatment: Rad
Tissue Type: CNS
Histology: Glioblastoma; left temporal; non epithelial
p53 Status: 0
ECAD Methylation: 7
MDR Function: 91
Institution: University of California
Contributor: Rosenblum, ML
Reference: Acta Neuropathol. 75:92-103, 1987; Cancer Res. 46:5893-5902, 1986
SF295 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
SF295 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on SF295 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SF295 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of SF295 Cell Avalanche® Transfection Reagent for transfection on SF295 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
 

Features:

• Unique formulation-maximize transfection performance in SF295 cells
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Free of animal-derived components
• Simplest and straightforward protocols to Increase experimental throughput
• Compatible with serum
• Suitable for Reverse Transfection  
• Compatible with transfection in any plate format
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SF295 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SF295 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SF295 Cell Avalanche® Transfection Reagent.   FIG. 2. SF295 cells were transfected with GFP vector (pEGFP-N3) by using SF295 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

SF295 Cell Avalanche® Transfection Reagent (human glioblastoma cell) can also be used on the following cells with high transfection efficiencies. SF268 Cell SF539 Cell U251 Cell SNB75 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell BHK-21 Cell 3197-3 Cell NIH/3T3 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200