Cell Line Information:

Designations: SK-BR-3
Depositors: G Trempe, LJ Old
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: mammary gland; breast
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion
Isolation date: 1970
Applications: transfection host
Tumorigenic: Yes
Antigen Expression: Blood Type A; Rh ; HLA A11, Bw22( /-), B40, B18
Age: 43 years
Gender: female
Ethnicity: Caucasian
Comments: The SK-BR-3 cell line overexpresses the HER2/c-erb-2 gene product. Ultrastructural features include microvilli and desmosomes, glycogen granules, large lysosomes, bundles of cytoplasmic fibrils.

The Transfection Reagent:

SK-BR-3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SK-BR-3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SK-BR-3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into SK-BR-3 Cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Efficient for plasmid DNA/siRNA co-transfection
• Simplest and straightforward protocols
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDA-MB-453 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDA-MB-453 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on SK-BR-3 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as SK-BR-3 Cell Avalanche Transfection Reagent.   FIG. 2. SK-BR-3 cells were transfected with GFP vector (pEGFP-N3) by using SK-BR-3 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells:

SK-BR-3 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies. 4T1 Cell ZR-75-1 Cell BT-20 Cell MDA-MB-453 Cell HCC1937 Cell MDA-MB-468 Cell MDA-MB-231 Cell 293 Cell 293T/17 Cell  Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200