Cell Line Information:
Organism: Homo sapiens (human)
Tissue: malignant melanoma; skin
Age: 51 years
Gender: male
Morphology: polygonal
Growth properties: adherent
Tumorigenic: yes, in nude mice; forms malignant melanoma (large round cell type)
Isoenzymes: PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1-2; GLO-1, 2; G6PD, B
AntigenExp: Blood Type A; Rh ; HLA A11, A26, B40, DRw4
Depositors: T. Takahashi

SK-MEL-28 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
The Transfection Reagent:

SK-MEL-28 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on SK-MEL-28 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SK-MEL-28 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of SK-MEL-28 Cell Avalanche™ Transfection Reagent for transfection on SK-MEL-28 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into SK-MEL-28 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A-375 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A-375 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in SK-MEL-28 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as SK-MEL-28 Cell Avalanche Transfection Reagent.   FIG. 2. SK-MEL-28 cells were transfected with GFP vector (pEGFP-N3) by using SK-MEL-28 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

SK-MEL-28 Cell Avalanche™ Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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