4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营类目:分子生物学酶,生物合成,分子试剂,生物耗材等
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > > Cambio/SK-MEL-5 Cell Avalanche® Transfection Reagent (human melanoma cell)/EZT-SME5-1-15
商品详细Cambio/SK-MEL-5 Cell Avalanche® Transfection Reagent (human melanoma cell)/EZT-SME5-1-15
Cambio/SK-MEL-5 Cell Avalanche® Transfection Reagent (human melanoma cell)/EZT-SME5-1-15
Cambio/SK-MEL-5 Cell Avalanche® Transfection Reagent (human melanoma cell)/EZT-SME5-1-15
商品编号: EZT-SME5-1-15
品牌: Cambio
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 其他
公司分类:
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description

Cell Line Information:
Cell Line Name: SK-MEL-5       (ATCC® HTB-70™)
Organism: Homo sapiens, human
Tissue: skin, derived from metastatic axillary node.
Cell Type: Melanoma
Morphology: stellate
Culture Properties: adherent
Biosafety Level: 1
Disease: malignant melanoma
Age: 24 years
Gender: female
Ethnicity: Caucasian
Markers: Blood Type O; Rh ; HLA A2, A11, B40, Bw16
Tumorigenic: Yes
Comments: This is one of a very extensive series of melanoma lines (see also ATCC HTB-71, ATCC HTB-72 and ATCC HTB-73) isolated by T. Takahashi and associates. The lines served as source of target cells for detection of melanoma specific antibody in patients with the disease.
Institution: The Memorial Sloan-Kettering Cancer Center
SK-MEL-5 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
SK-MEL-5 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SK-MEL-5 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SK-MEL-5 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?


Features:

  • Unique formulation-maximize transfection performance in SK-MEL-5 cells
  • Extremely gentle to cells
  • Deliver single or multiple plasmids
  • Free of animal-derived components
  • Simplest and straightforward protocols to Increase experimental throughput
  • Active in serum-containing medium
  • Suitable for Reverse Transfection  
  • Compatible with transfection in any plate format
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Great pricing
  • Developed and manufactured by EZ Biosystems
 
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SK-MEL-5 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SK-MEL-5 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SK-MEL-5 Cell Avalanche® Transfection Reagent.
 

For Other Cells:
SK-MEL-5 Cell Avalanche® Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies.

LOX-IMVI Cell  
M14 Cell
SK-MEL-2 Cell
UACC-257 Cell  
UACC-62 Cell  
A-375 Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.


If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

品牌介绍
CambioCambio成立于1985年,是一家私人控股的英国公司,在为大学,大型制药,生物技术,政府机构,农业组织和环境设施的科研实验室提供优质分子生物学试剂和消耗品方面享有盛誉。Established in 1985, Cambio is a privately held UK company with an excellent reputation for the supply of quality Molecular Biology reagents and consumables to scientific research laboratories within universities, large pharma, biotechs, government agencies, agricultural organisations and environmental facilities.产品列表:No.品牌货号名称规格1cambioCA-1188A2-01 RRUCL2Anti Human Collagenase, monoclonal mouse0.1mg2cambioCA-1188-BS1Anti Human TIMP-1, polyclonal Rabbit0.1mg3cambioCA-1012Anti Streptokinase, polyclonal sheep1ml4cambioCA-605Anti Human Urokinase, polyclonal goat1ml5cambioCA-1046A c-terminusAnti Human Interleukin 5, polyclonal rabbit1ml6cambioCA-1075A c-terminusAnti Human Dopamine D2-3 Receptor, polyclonal rabbit1ml7cambioCA-1119Anti Human Cystic Fibrosis Protein, polyclonal rabbit1ml8cambioCA-1511BAnti MMP9 (Gelatinase B), polyclonal rabbit1 ml9cambioCA-972-FHuman Placental DNA (intact genomic) 1mg 10cambioCA-972-MHuman Placental DNA (intact genomic)1mg 11cambioCA-972-06Human Genomic DNA (sonicated)100mg12cambioPM-1025-01Alkaline Phosphatase1,000U13cambioPM-1025-02Alkaline Phosphatase5000U14cambioCA-972-FHuman Placental DNA (intact genomic)1mg15cambioCA-972-MHuman Placental DNA (intact genomic)1mg