Cell Line Information:

Designations: SK-OV-3
Organism: Homo sapiens (human)
Tissue: ovary; adenocarcinoma; derived from metastatic site: ascites
Age: 64 years
Gender: female
Ethnicity: caucasian
Morphology: epithelial
Growth Properties: adherent
Isoenzymes: PGM3, 1; PGM1, 1-2; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0311
Antigen Expression: Blood Type B; Rh
Tumorigenic: yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with ovarian primary
Depositors: G. Trempe; Lloyd J. Old
SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis-platinum and adriamycin.

SK-OV-3 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

SK-OV-3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SK-OV-3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SK-OV-3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into SK-OV-3 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Transfection Performance

• Transfection efficiency up to 91%
• Viability up to 95%

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SK-OV-3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SK-OV-3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SK-OV-3 Cell Avalanche Transfection Reagent.   FIG. 2. SK-OV-3 cells were transfected with GFP vector (pEGFP-N3) by using SK-OV-3 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

SK-OV-3 Cell Avalanche™ Transfection Reagent (human ovary carcinoma cell) can also be used on the following cells with high transfection efficiencies. CHO-K1 Cell Vero Cell 293 Cell 293T/17 Cell Saos-2 Cell B16-F10 Cell 4T1 Cell A549 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200