Cell Line Information:
Designations: Sp2/0-Ag14
Organism: Mus musculus mouse (B cell); mouse (myeloma)
Strain: BALB/c (B cell); BALB/c (myeloma)
Tissue: B lymphocyte; hybridoma
Morphology: lymphoblast
Growth properties: suspension
Depositor: G. Kohler
AntigenExp: H-2
The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 mcg/ml and are HAT sensitive. Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas.

The Transfection Reagent:

Transfection of suspension cells, such as Sp2/0-Ag14 Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.

Sp2/0-Ag14 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Sp2/0-Ag14 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Sp2/0-Ag14 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of Sp2/0-Ag14 Cell Avalanche™ Transfection Reagent for transfection on Sp2/0-Ag14 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into Sp2/0-Ag14 Cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SP2/0-AG14 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SP2/0-AG14 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SP2/0-AG14 Cell Avalanche Transfection Reagent.

For Other Cells:

Sp2/0-Ag14 Cell Avalanche™ Transfection Reagent (mouse hybridoma cell) can also be used on the following cells with high transfection efficiencies. Vero Cell 293 Cell 293T/17 Cell MG-63 Cell WI-38 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200