Cell Line Information:
Cell Line Name: SR (ATCC® CRL-2262™)
Organism: Homo sapiens, human
Tissue: pleural effusion
Cell Type: lymphoblast
Morphology: lymphoblast
Culture Properties: suspension
Disease: large cell immunoblastic lymphoma
Age: 11 years
Gender: male
Ethnicity: Caucasian
Institution: Program Resources, Inc.
Comments Derivation: SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo. SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages. Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition. SR cells have been reported to be Epstein-Barr virus genome negative.
SR is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
 
The Transfection Reagent:

Transfection of suspension cells, or suspension/adherent mixed cells, such as SR cell, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect suspension cells, as well as suspension/adherent mixed cells.

SR Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on SR cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SR cells. No other transfection reagents can match the efficiency, convenience, and gentleness of SR Cell Avalanche® Transfection Reagent for transfection on SR cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
 

Features:

• Specifically optimized to deliver nucleic acids into SR cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols to Increase experimental throughput
• Compatible with serum
• Suitable for Reverse Transfection  
• Compatible with transfection in any plate format
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SR cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SR cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SR Cell Avalanche® Transfection Reagent.

For Other Cells:

SR Cell Avalanche® Transfection Reagent (human lymphoma cell) can also be used on the following cells with high transfection efficiencies. M14 Cell SK-MEL-2 Cell UACC-62 Cell   A-375 Cell SK-MEL-28 Cell MDCK Cell Vero Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200