Cell Line information:

Designations: SW480 [SW-480]
Depositors: A Leibovitz
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Tumor Stage: Dukes" type B
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA) 0.7 ng/10 exp6 cells/10 days; keratin; transforming growth factor beta
Applications: transfection host
Receptors: epidermal growth factor (EGF)
Virus Susceptibility: Human immunodeficiency virus 1
Tumorigenic: Yes
Antigen Expression: HLA A2, B8, B17; blood type A; Rh
Age: 50 years
Gender: male
Ethnicity: Caucasian
Comments: SW480 was established from a primary adenocarcinoma of the colon. The line is negative for CSAp (CSAp-) and colon antigen 3, and is positive for keratin by immunoperoxidase staining. The cells express elevated levels of p53 protein. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. The cells have been reported to produce GM-CSF.

The Transfection Reagent:

SW480 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SW480 Cells.  The proprietary formulation of lipids and polymers ensures the maximum possible transfection efficiency and cell viability. No other transfection reagents can match the efficiency, convenience, and gentleness of SW480 Cell Avalanche™ Transfection Reagent for transfection on SW480 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in SW480 Cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Efficient for plasmid DNA/siRNA co-transfection
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T84 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for T84 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on SW480 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as SW480 Cell Avalanche Transfection Reagent.

For Other Cells:

SW480 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. T84 Cell HCT 116 Cell DLD-1 Cell HT-29 Cell Caco-2 Cell LS 174T Cell LoVo Cell SW620 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200