Cell Line Information:
Cell Line Name: T47D          (ATCC® HTB-133™)
Organism: Homo sapiens, human
Tissue: mammary gland; breast/duct: pleural effusion
Cell Type: epithelial transfected with reporter plasmid
Morphology:    epithelial
Culture Properties: adherent
Disease: ductal carcinoma
Age: 54 years
Gender: female
Contributor: Keydar, I
Reference: Eur J Cancer. 15(5):659-70, 1979
Applications: This cell line can be used to screen chemicals for estrogenic or anti-estrogenic activity.
T47D is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
T47D Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on T47D cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for T47D cells. No other transfection reagents can match the efficiency, convenience, and gentleness of T47D Cell Avalanche® Transfection Reagent for transfection on T47D cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
 

Features:

• Unique formulation-maximize transfection performance in T47D cells
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Active in serum-containing medium
• Suitable for Reverse Transfection  
• Compatible with transfection in any plate format
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Great pricing
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T47D cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for T47D cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this T47D Cell Avalanche® Transfection Reagent.   FIG. 2. T47D cells were transfected with GFP vector (pEGFP-N3) by using T47D Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells:

T47D Cell Avalanche® Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies. BT-20 Cell HCC1937 Cell MCF7 cell Cell Hs-578-T Cell MDA-MB-468 Cell SK-BR-3 Cell MDA-MB-231 Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200