ell Line Information:

Designations: T84
Depositors: H Masui
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Disease: colorectal carcinoma
Derived from metastatic site: lung
Cellular Products: carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin
Applications: transfection host
Receptors: neurotransmitter, peptide hormone
Tumorigenic: Yes
Age: 72 year
Gender: male
Comments: The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells. They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport. This line exhibits tight junctions, and desmosomes between adjacent cells. The cells are positive for keratin by immunoperoxidase staining.

The Transfection Reagent:

T84 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on T84 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for T84 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of T84 Cell Avalanche™ Transfection Reagent for transfection on T84 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in T84 Cells.
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Efficient for plasmid DNA/siRNA co-transfection
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T84 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for T84 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this T84 Cell Avalanche Transfection Reagent.

For Other Cells:

T84 Cell Avalanche™ Transfection Reagent (human colon carcinoma cell) can also be used on the following cells with high transfection efficiencies. HCT 116 Cell DLD-1 Cell HT-29 Cell Caco-2 Cell LS 174T Cell LoVo Cell SW480 Cell SW620 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200