Cell Line Information:
Cell Line Name: TK10, TK.10
Patient"s Age: 43
Patient"s Sex: M
Prior Treatment: No
Tissue Type: Kidney
Histology: Renal cell adenocarcinoma; primary; epithelium
p53 Status: 0
ECAD Methylation: 59
MDR Function: -4
Institution: Washington University School of Medicine
Contributor: Clayman, RV
Reference: Cancer Res. 47:3856-3862, 1987
TK10 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
TK10 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting TK10 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for TK10 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into TK10 cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols to Increase experimental throughput
• Active in serum-containing medium
• Suitable for Reverse Transfection  
• Compatible with transfection in any plate format
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on TK10 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for TK10 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this TK10 Cell Avalanche® Transfection Reagent.

For Other Cells:

TK10 Cell Avalanche® Transfection Reagent (human kidney adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. A498 Cell   ACHN Cell   RXF393 Cell   SN12C Cell   UO31 Cell   Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200