Cell Line Information:
Designations: U266B1
Organism: Homo sapiens (human)
Tissue: B lymphocyte; plasmacytoma; myeloma
Age: 53 years
Gender: male
Cellular products: immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6)
Isotype: IgE; lambda light chain
Morphology: lymphoblast
Growth properties: suspension
Depositors: N.K. Nilsson
The U266 cell line was established from the peripheral blood of a patient with an IgE myeloma (epsilon2, lambda2). These cells secrete IgE. Immunological studies indicate that the U266 cell line was derived from the same clone of myeloma cells which grew in vivo since the immunoglobulin synthesized in vitro by the cell line is identical to the myeloma protein produced in vivo. U266 cells have been reported to produce human IL-6.

The Transfection Reagent:

Transfection of suspension cells, such as U266B1 Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.

U266B1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting U266B1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for U266B1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on U266B1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for U266B1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this U266B1 Cell Avalanche Transfection Reagent.

For Other Cells:

U266B1 Cell Avalanche™ Transfection Reagent (human myeloma cell) can also be used on the following cells with high transfection efficiencies. Vero Cell 293 Cell 293T/17 Cell MG-63 Cell WI-38 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200