Cell Line Information:

Designations: ZR-75-1
Depositors: LW Engel
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: mammary gland; breast
Tissue: duct
Disease: ductal carcinoma
Derived from metastatic site: ascites
Cell Type: epithelial
Cellular Products: mucin (apomucin, MUC-1, MUC-2)
Receptors: estrogen receptor, expressed
Tumorigenic: Yes
Isoenzymes: G6PD, B
Age: 63 years adult
Gender: female
Ethnicity: Caucasian
Comments: The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.

The Transfection Reagent:

ZR-75-1 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on ZR-75-1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for ZR-75-1 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of ZR-75-1 Cell Avalanche™ Transfection Reagent for transfection on ZR-75-1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in ZR-75-1 Cells.
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on ZR-75-1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for ZR-75-1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this ZR-75-1 Cell Avalanche Transfection Reagent.   FIG. 2. ZR-75-1 cells were transfected with GFP vector (pEGFP-N3) by using ZR-75-1 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells:

ZR-75-1 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies. 4T1 Cell BT-20 Cell MDA-MB-453 Cell HCC1937 Cell MDA-MB-468 Cell SK-BR-3 Cell MDA-MB-231 Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200