Cell Information:

Although lymphatic micorvascular endothelial cells have many properties in common with blood microvascular endothelial cells, they also have unique functions due to their structural features. Lymphatic capillaries have thin and discontinuous base membrane and are directly connected to the insterstitial collagens by elastic anchoring filaments. The endothelial cells of lymphatic vessel wall loosely overlap to form easily opened minivalves that make them highly permeable. Recent studies demonstrate that tumor lymphangiogenesis is a major component of the metastatic process and members of the VEGF family are key mediators of lymphangiogenesis in tumors.
Human Dermal Lymphatic Microvascular Endothelial (HDLMVEC) are usually isolated from lymphatic capillaries of normal human dermis. HDLMVEC could be used for the following applications:

• Dermal microvasculature pathology
• Inflammation
• Oncology
• Diabetes
• Pulmonary disease/disorders
• Drug toxcity

The Transfection Reagent:

Transfection of primary cells, such as HDLMVEC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Dermal Lymphatic Microvascular Endothelial Cell (HDLMVEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HDLMVEC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HDLMVEC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HDLMVEC
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human lung microvascular endothelial cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human lung microvascular endothelial cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested in human dermal lymphatic microvascular endothelial cell (HDLMVEC) by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Human Dermal Lymphatic Microvascular Endothelial Cell (HDLMVEC) Avalanche Transfection Reagent.

For Other Cells:

Human Dermal Lymphatic Microvascular Endothelial Cell (HDLMVEC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. Human Umbilical Vein Endothelial Cell (HUVEC) Human Umbilical Artery Endothelial Cell (HUAEC) Human Dermal Microvascular Endothelial Cell (HMVEC) Human Artery Endothelial Cell Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200