Cell Information:

Human Fibroblast-like synoviocytes (HFLS) represent a specialised cell type located inside joints in the synovium. HFLS are long known for their role in joint destruction by producing cytokines and metalloproteinases that contribute to cartilage degradation. Proinflammatory cytokines induce the proliferation, collagenase and aggrecanase production and GM-CSF secretion on HFLS. Ongoing arthritis research also has shown that HFLS express apoptosis and P53 mutations

Human Fibroblast-Like Synoviocytes (HFLS) are usually isolated from normal synovial tissues. These cells can be used as a good in vitro model for studying the pathogenesis of chronic inflammatory diseases, such as rheumatoid arthritis.

The Transfection Reagent:

Transfection of primary cells, such as HFLS, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Fibroblast-Like Synoviocyte (HFLS, HFLS-OA, HFLS-RA) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HFLS. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HFLS. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Fibroblast-Like Synoviocyte (HFLS, HFLS-OA, HFLS-RA) Avalanche™ Transfection Reagent for transfection on HFLS. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human fibroblast-like synoviocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human fibroblast-like synoviocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Fibroblast-Like Synoviocyte Avalanche Transfection Reagent.

For Other Cells:

Human Fibroblast-Like Synoviocyte (HFLS, HFLS-OA, HFLS-RA) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. BALB/3T3 clone A31 3T3-Swiss albino Cell CT26.WT Cell Human Dermal Fibroblast (HDF) Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF) Human Lung Fibroblast (HLF, NHLF) 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200