Cell Information:

HLMVEC and elevated expression of chemoattractant cytokines are critical for directed migration of leucocytes into the lung during the induction and perpetuation of acute lung injury. Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro.

Applications:

• Vascular pathology
• Inflammation
• Oncology
• Pulmonary hypertension
• Pulmonary disease/disorders
• Drug toxcity

The Transfection Reagent:

Transfection of primary cells, such as HLMVEC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HLMVEC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HLMVEC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HLMVEC
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

 Data:FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human lung microvascular endothelial cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human lung microvascular endothelial cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Lung Microvascular Endothelial Cell Avalanche Transfection Reagent.
For Other Cells:Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

Human Umbilical Vein Endothelial Cell (HUVEC)
Human Umbilical Artery Endothelial Cell (HUAEC)
Human Dermal Microvascular Endothelial Cell (HMVEC)
Human Dermal Lymphatic Microvascular Endothelial Cell (HDLMVEC)
Human Artery Endothelial Cell
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200