Cell Information:

Human Mammary Epithelial Cells (HMEpC) are usually derived from normal adult mammary glands. It was shown that normal HMEpC have a high amount of cyclin-dependent kinase 6 activity, which restrains HMEpC proliferation, and loss or down-regulation of cdk 6 may play a role in breast tumor development1. Transforming HMEpC to immortality and malignancy was a useful model for studying early breast carcinogenesis. Autocrine colony-stimulating factor receptors may contribute to invasive progression in epithelial cancer was demonstrated in a 3-dimensional culture of HMEpC.

Applications:

• Breast cancer
• Cellular function and differentiation
• Physiology
• Toxicology
• Hormone regulation 

The Transfection Reagent:

Transfection of primary cells, such as HMEpC, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Mammary Epithelial Cell (HMEpC, HMEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HMEpC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HMEpC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HMEpC
• Extremely gentle to cells
• Deliver single or multiple plasmids
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human mammary epithelial cell (HMEpC) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human mammary epithelial cell (HMEpC). This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Mammary Epithelial Cell (HMEpC) Avalanche Transfection Reagent.

For Other Cells:

Human Mammary Epithelial Cell (HMEpC, HMEC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. Human Urethra Epithelial Cell (HUEpC) Human Renal Epithelial cell (HRE) Human Cervical Epithelial Cell (HCvEpC) Human Bladder Epithelial Cell (HBlEpC) Human Placental Epithelial Cell (HPlEpC) 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200