Cell Information:

Human Osteoblasts (HOb) sythesize bone matrix. Matrix mineralization can be induced in the presence of ascorbic acid and B-Glycerophosphate. Hob are isolated from normal human bone. These cells are commonly used for micro- and macroscopic investigations of the skeletal system, such as the effects of inflammatory mediators, the effects of bone morphogenic proteins, the physiological control of bone remodeling, the regulation of bone metabolism, and the study of the chemicals associated with physical trauma.

Applications:

• In vitro bone models
• Mineralization
• Osteoporosis
• Gene expression
• Hormone treatments
• Osteopenia
• Paget"s Disease

The Transfection Reagent:

Transfection of primary cells, such as HOb, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Osteoblast (HOb, HOb-OA, HOb-RA, NHOst) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HOb. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HOb. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Osteoblast (HOb, HOb-OA, HOb-RA, NHOst) Avalanche™ Transfection Reagent for transfection on HOb. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

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