Cell Information:

Human Placental Epithelial Cells (HPlEpC) are usually isolated from normal human amnion. HPlEpC has been used to study the secretion of activin A, the neural formation during early fetal development, and the antimicrobial and endotoxin-neutralizing barrier effect of Histones H2A and H2B against microorganism. Prostaglandin E2 induces high level of amniotic epithelial cell death via necrosis rather than apoptosis. Matrix metalloproteinases?9 (MMP-9) localized mainly in amnion epithelia. An increase in MMP-9 expression may contribute to degradation of extracellular matrix in fetal membrane and placenta, thereby facilitate fetal membrane rupture and placental detachment.

The Transfection Reagent:

Transfection of primary cells, such as HPlEpC, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Placental Epithelial Cell (HPlEpC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HPlEpC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HPlEpC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Placental Epithelial Cell (HPlEpC) Avalanche™ Transfection Reagent for transfection on HPlEpC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in HPlEpC.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human placental epithelial cell (HPlEpC) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human placental epithelial cell (HPlEpC). This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Placental Epithelial Cell (HPlEpC) Avalanche Transfection Reagent.

For Other Cells:

Human Placental Epithelial Cell (HPlEpC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. Human Urethra Epithelial Cell (HUEpC) Human Renal Epithelial cell (HRE) Human Cervical Epithelial Cell (HCvEpC) Human Bladder Epithelial Cell (HBlEpC) Human Mammary Epithelial Cell (HMEpC, HMEC) 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200