Cell Information:

Human Preadipocytes (HPAd) are derived from human adipose tissue. These fibroblast-like precursor cells can be differentiated into Human Adipocytes (HAd). HPAd derived HAd were used to study insulin-stimulated glucose transport, growth hormone enhanced lypolysis and obese gene expression. Significant differences in lipolysis and leptin production are observed in HPAd and HAd from human omental and subcutaneous adipose tissue. HPAd/HAd are a useful in vitro cellular model for investigating the causes and treatment of obesity, type II diabetes and cardiovascular diseases. Recently it was discovered that human adipocytes might be a new target for the immunodeficiency virus.

The Transfection Reagent:

Transfection of primary cells, such as HPAd, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Preadipocyte (HPAd) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HPAd. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HPAd. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Preadipocyte (HPAd) Avalanche™ Transfection Reagent for transfection on HPAd. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HPAd
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

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3T3-F442A
Ob17  
AP-18
BJ Cell
Detroit 551 Cell
MG-63 Cell
Human Dermal Fibroblast (HDF)
Human Cardiac Fibroblasts (HCF, NHCF-A, NHCF-V, AoAF)
Human Lung Fibroblast (HLF, NHLF)  
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293 Cell
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