Cell Information:

Prostate cells provide a glandular function in the body by generating fluid which serves several functions in reproduction. Prostate secretions get into the urethra through contraction of the smooth muscle cells (PrSMC). PrSMC can be involved in prostitis and benign prostatic hyperplasia (BPH). 
PrSMC can be used for the following applications:

• Benign prostatic hyperplasia (BPH)
• Prostitis
• Prostate cancer
• Procreation research
• Drug discovery
• Male prostate physiology

The Transfection Reagent:

Transfection of primary cells, such as PrSMC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Prostate Smooth Muscle Cell (PrSMC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on PrSMC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PrSMC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Prostate Smooth Muscle Cell (PrSMC) Avalanche™ Transfection Reagent for transfection on PrSMC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in PrSMC.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human aortic smooth muscle cell (HAOSMC) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HAOSMC. This screening result was confirmed with repeat experiments. The candidate formulas were also tested in human prostate smooth muscle cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Human Prostate Smooth Muscle Cell Avalanche Transfection Reagent.

For Other Cells:

Human Prostate Smooth Muscle Cell (PrSMC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. Human Umbilical Vein Smooth Muscle Cell (HUVSMC) Human Artery Smooth Muscle Cell 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200