Cell Information:

Human Skeletal Muscle Cells (HSkMC) usually are isolated from the limbal skeletal muscle. HSkMC can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC have been used in the study of genetic muscular diseases such as Malignant Hyperthermia. HSkMC also have the potential to act as a cardiac graft, mending damage to the heart. Recently, cultured HSkMC have been used in micro gravity experiments to study the effects of low gravity environments on Human Skeletal Muscle.

Human Skeletal Muscle Myoblasts (HSMMs) usually are isolated from post-gestational tissue usually from the quadriceps or psoas tissue. HSMMs can be differentiated to form myotubes in culture.

Applications:

• Cellular development
• Differentiation
• Diabetes
• Insulin uptake or resistance
• Tissue repair
• Drug development
• Myotube development
• Co-culture with other cell types

The Transfection Reagent:

Transfection of primary cells, such as Human Skeletal Muscle Cells and Myoblasts, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Skeletal Muscle Cell and Myoblast (HSkMC, SkMC, HSMM) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on Human Skeletal Muscle Cells and Myoblasts. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Skeletal Muscle Cells and Myoblasts. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Skeletal Muscle Cell and Myoblast (HSkMC, SkMC, HSMM) Avalanche™ Transfection Reagent for transfection on Human Skeletal Muscle Cells and Myoblasts. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into Human Skeletal Muscle Cells and Myoblasts
• Extremely gentle to cells
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Suitable for Reverse Transfection 
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems 

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human skeletal muscle cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human skeletal muscle cell. This test result was confirmed by transfecting human skeletal myoblasts mentioned above. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Skeletal Muscle Cell and Myoblast Avalanche Transfection Reagent.

For Other Cells:

Human Skeletal Muscle Cell and Myoblast (HSkMC, SkMC, HSMM) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200