Cell Information:

Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. HUVEC are responsive to cytokine stimulation in the expression of cell adhesion molecules. These cell systems are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, and fibrinolysis. HUVEC can be used in the following applications:

• Cardiovascular pharmaceutical development
• Vascular pathology
• Atherosclerosis
• Angiogenesis
• Inflammation
• Wound healing
• Oncology

The Transfection Reagent:

Transfection of primary cells, such as HUVEC, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Umbilical Vein Endothelial Cell (HUVEC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HUVEC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HUVEC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Umbilical Vein Endothelial Cell (HUVEC) Avalanche™ Transfection Reagent for transfection on HUVEC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HUVEC
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. (Click image to enlarge) High throughput test of transfection efficiency (determined as RLU/mg) on human umbilical vein endothelial cells (HUVEC) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HUVEC. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Umbilical Vein Endothelial Cell (HUVEC) Avalanche Transfection Reagent.   FIG. 2. (Click image to enlarge) HUVEC were transfected with GFP vector (pEGFP-N3) by using Lipofectamine® 3000 and HUVEC Avalanche® Transfection Reagent resptctively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection. In addition to higher transfection efficiency (% GFPcells) and higher expression level (brighter) as compared to those of Lipofectamine® 3000, HUVEC Avalanche® Transfection Reagent also shows the least toxicity to the cells. From this figure, HUVEC Avalanche® transfected cells look more healthy (variety of shapes, contact with each other), whereas Lipofectamine® 3000 transfected cells show some signs of stress (smaller, separate from each other, and with vacuoles or bubbles in the cytoplasm). Lipofectamine® is a trademark of Life Technologies Corporation. Avalanche® is a trademark of EZ Biosystems LLC

For Other Cells:

Human Umbilical Vein Endothelial Cell (HUVEC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. Human Umbilical Artery Endothelial Cell (HUAEC) Human Dermal Microvascular Endothelial Cell (HMVEC) Human Dermal Lymphatic Microvascular Endothelial Cell (HDLMVEC) Human Artery Endothelial Cell Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200