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当前位置： 首页 > 产品中心 > > Cambio/Avalanche-PRO Transfection Reagent/EZT-PROT-1-150
商品详细Cambio/Avalanche-PRO Transfection Reagent/EZT-PROT-1-150
Cambio/Avalanche-PRO Transfection Reagent/EZT-PROT-1-150
Cambio/Avalanche-PRO Transfection Reagent/EZT-PROT-1-150
商品编号： EZT-PROT-1-150
品牌： Cambio
市场价： ¥0.00
美元价： 0.00
产地： 美国(厂家直采)
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产品分类： 其他
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联系Q Q： 3392242852
电话号码： 4000-520-616
电子邮箱： info@ebiomall.com
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商品介绍

Description

Avalanche®-PRO Transfection Reagent is a proprietary, animal origin-free formulation of lipids and polymers for transfecting plasmid DNA into eukaryotic cells that can easily be scaled up to produce large amounts of recombinant proteins. Avalanche®-PRO Transfection Reagent allows the highest expression levels and transfection rates with lowest cytotoxicity in bio-production applications, and is specifically formulated for use with suspension 293 and CHO derived cells, such as FreeStyle 293-F Cells (suspension human embryonal kidney cells, Life Technologies, Cat. no. R790-07), FreeStyle CHO-S Cells (suspension Chinese Hamster Ovary cells, Life Technologies, Cat. no. R800-07), and DG44 Cells (DHFR- suspension CHO cells).

Features:

  • Highest recombinant protein yield
  • Lesser  plasmid DNA and Transfection Reagent needed
  • Compatible with multiple CHO media formulations
  • Proven Scalability
  • Simplest transfection protocol
  • Totally animal free
  • Half cost and similar or higher performances per reacton as compared to FreeStyle™ Max Reagent.

 

In brief, Avalanche®-PRO Transfection Reagent is ideal for Biotherapeutic Protein Production

 
Data:
Figure 1. Lesser DNA and lesser transfection reagent are needed for Avalanche®-PRO Transfection Reagent to have similar performances as compared to FreeStyle Max Reagent. (A) FreeStyle 293-F Cells were cultured in 30 ml of FreeStyle 293 Expression Medium to a density of 1 x 106 cells/ml for the transfection of pCMV·SPROT beta-gal as plasmid DNA. For Avalanche®-PRO Reagent transfection, DNA and Transfection Reagent used are 30 µg and 24 µl respectively; For FreeStyle Max Reagent transfection, DNA and Transfection Reagent used are 37.5µg and 37.5 µl respectively according to the respective protocols. (B) FreeStyle™ CHO-S Cells were cultured in 30 ml of FreeStyle™ CHO Expression Medium to a density of 1 x 106 cells/ml for the transfection of human IgG plasmid DNA. For Avalanche®-PRO Reagent transfection, DNA and transfection reagent used were 30 µg and 30 µl respectively; For FreeStyle Max Reagent transfection, DNA and Transfection Reagent used are 37.5µg : 37.5 µl respectively according to the respective protocols.
 
Figure 2. Avalanche®-PRO provides high performance across varied media formulations. FreeStyle™ CHO-S cells were adapted to 8 representative growth media including: ProCHO™5 medium (Lonza Inc.), PowerCHO 2 CD medium (Lonza Inc), HyCell* CHO Medium (Thermo Scientific), FreeStyle™ CHO Expression medium (Life Technologies), Cellvento™ CHO-100 (EMD Millipore ), BD Select™ CHO medium (BD Biosciences), BD Select™ CD1000 medium (BD Biosciences), and EX-CELL® CD CHO-2 Medium (Sigma-Aldrich). Cells were transfected with a human IgG plasmid DNA using Avalanche-PRO (reagent:DNA=1.0 µl:1.0 µg/ml culture), or FreeStyle Max (Life Technologies) (reagent:DNA=1.25 µl:1.25 µg/ml culture) transfection reagents according to the published protocol ). Transfections were performed in 24-well deep well shaker blocks using 1 x 106 cells/ml at the time of transfection. Human IgG was quantitated from day 6 clarified supernatants and. Error bars represent the standard deviation of triplicate wells.
 
Figure 3. Avalanche®-PRO Transfection Reagent Scalability and Cost Saving as compared to FreeStyle Max Reagent. (A)Avalanche®-PRO transfections in culture volumes from 1 ml to 250 ml resulted in consistant Human IgG yield in terms of µg/ml culture. (B) and (C): While the transfection performance of Avalanche®-PRO Transfection Reagent is similar to that of FreeStyle MAX Reagent, the cost of Avalanche®-PRO needed per reacton is only half of the cost of FreeStyle MAX Reagent needed.

 

 


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品牌介绍
CambioCambio成立于1985年，是一家私人控股的英国公司，在为大学，大型制药，生物技术，政府机构，农业组织和环境设施的科研实验室提供优质分子生物学试剂和消耗品方面享有盛誉。Established in 1985, Cambio is a privately held UK company with an excellent reputation for the supply of quality Molecular Biology reagents and consumables to scientific research laboratories within universities, large pharma, biotechs, government agencies, agricultural organisations and environmental facilities.产品列表：No.品牌货号名称规格1cambioCA-1188A2-01 RRUCL2Anti Human Collagenase, monoclonal mouse0.1mg2cambioCA-1188-BS1Anti Human TIMP-1, polyclonal Rabbit0.1mg3cambioCA-1012Anti Streptokinase, polyclonal sheep1ml4cambioCA-605Anti Human Urokinase, polyclonal goat1ml5cambioCA-1046A c-terminusAnti Human Interleukin 5, polyclonal rabbit1ml6cambioCA-1075A c-terminusAnti Human Dopamine D2-3 Receptor, polyclonal rabbit1ml7cambioCA-1119Anti Human Cystic Fibrosis Protein, polyclonal rabbit1ml8cambioCA-1511BAnti MMP9 (Gelatinase B), polyclonal rabbit1 ml9cambioCA-972-FHuman Placental DNA (intact genomic) 1mg 10cambioCA-972-MHuman Placental DNA (intact genomic)1mg 11cambioCA-972-06Human Genomic DNA (sonicated)100mg12cambioPM-1025-01Alkaline Phosphatase1,000U13cambioPM-1025-02Alkaline Phosphatase5000U14cambioCA-972-FHuman Placental DNA (intact genomic)1mg15cambioCA-972-MHuman Placental DNA (intact genomic)1mg
公司介绍
CambioCambio成立于1985年，是一家私人控股的英国公司，在为大学，大型制药，生物技术，政府机构，农业组织和环境设施的科研实验室提供优质分子生物学试剂和消耗品方面享有盛誉。Established in 1985, Cambio is a privately held UK company with an excellent reputation for the supply of qua
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目录适体 Laryngopharyngeal Sampling Swab Resins sbeadex™ plant IMAC Agaroses Click Chemistry STEM Kits Broad Range Transfection Reagents sbeadex™ forensic
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